aflatoxın m1 kıt

Aflatoxin M1 Analysis

Aflatoxin M1 (AFM1) is the hydroxylated metabolite of AFB1. For the first time, AFM1 was found in the milk of lactating animals that consume feedstuffs contaminated with AFB1 in the 1960s. For AFM1, the Scientific Committee for Food concluded that there is sufficient evidences that aflatoxin notoxic carcinogen; its carcinogenic potency is estimated to be approximately 10 times lower than aflatoxin B1.

The European Community prescribes that the maximum level of AFM1 in liquid milk should not exceed 0.05 ppb (European Commission 2001). Such low limits require highly sensitive methods for the detection and quantification of AFM1 in milk. Jasem technology focuses on improvement of the sample preparation part, since this analysis-part is till now taking dis-proportional long time in existing reference & in house methods.

  • Features

    • No need for immunoaffinity column extraction
    • Very easy sample preparation
    • Shorter run time
    • No source contamination
  • Parameters

    Aflatoxin M1
  • MatrIx

    • Milk
    • Milk Powder
    • Infant Powder
    • Infant Milk Powder

Sample Preparation for Milk, Milk Powder, Infant Powder and Infant Milk Powder


  • Step 1

    Weigh 1.0 g of infant powder into a 50 ml polypropylene centrifuge tube

  • Step 2

    Add 10 ml of water and dissolve the powder by vortexing

  • Step 3

    Pipet 1.0 ml from the 10 ml milk powder solution into a glass centrifuge tube

  • Step 4

    Add 4.0 ml of Reagent-1 into the centrifuge tube and vortex for 15 seconds

  • Step 5

    Centrifuge at 3000 rpm for 4 minutes afterwards filter supernatant from 0.45 micron nylon filter into a HPLC vial

  • Example Chromatogram

  • Lınearıty & Quantıtatıon Lımıt

     

    Matrix

    R2

    LOQ (ng/ml)

    Goat Milk

    0.995

    0.017

    Infant Milk

    0.996

    0.015

    Infant Powder

    0.996

    0.013

  • Recovery

    Concentration

    Goat Milk

    Infant Milk

    Infant Powder

    (ng/ml)

    Recovery (%)

    Recovery (%)

    Recovery (%)

    0.0125

    100

    108

    98

    0.05

    117

    108

    108

    0.075

    108

    106

    106

  • Repeatabılıty

     

    Matrix

    RSD %

    Low conc(0,0125 ng/ml)

    Medium conc(0,05 ng/ml)

    High conc(0,075 ng/ml)

    Goat Milk

    5.05

    3.04

    2.21

    Infant Milk

    2.82

    3.80

    3.14

    Infant Powder

    6.54

    6.00

    4.18

  • ReproducIbIlIty

    Matrix

    RSD %

    Low conc(0,0125 ng/ml)

    Medium conc(0,05 ng/ml)

    High conc(0,075 ng/ml)

    Goat Milk

    7.51

    1.86

    1.21

    Infant Milk

    6.02

    4.90

    2.44

    Infant Powder

    7.73

    4.69

    1.61

ADAPTING DIAGNOSTICS IN CHROMATOGRAPHY COUPLED MASS SPECTROMETRY.

Contact Us

The easiest way is to fill the contact form. Simply mail us to info@jasem.com.tr or give us a call +902165720200