About

Quantitative Amino Acids Analysis

Analysis of free amino acids have applications of various fields, at the beginning of this diagnosis of inherited metabolic disorders, feeding newborn with a metabolic disorder inherited comes from studies. Furthermore, amino acid analysis in diagnosis problems such as liver and kidney disorders, intestinal microflora disorder, nov problems are performed. 43 amino acids are performed at 7.5 minutes with Jasem quantitative amino acids analysis kit for only one injection. Serum / plasma and urine samples are becoming ready for analysis at 5 min.

Maple-syrup urine disease (MSUD) is an inborn error of metabolism characterized by the inherited deficiency of the branched-chain-2-oxoacid dehydrogenase complex which is required in the metabolism of the branched-chain amino acids. The detection of allo-isoleucine allows for positive identification of patients with MSUD reducing the false-positive neonatal screening results. Chromatographic separation of allo-isoleucine is readily achieved in 11 minutes analysis time with the accurate quantification using same sample.

  • Features

    • Allows analysis without derivatization
    • Fast and simple preparation, no need SPE
    • Long lifetime HPLC column
    • Shorter analysis time (Analysis time  7.5 min)
    • Allow analysis allo-isoleucine for maple syrup disease diagnosis with only second injection (Analysis time 11 min)
  • Parameters

    Quantitative Amino Acids Analysis

    Alanine, arginine, aspartic acid, asparagine, 2-amino-butyric acid, argininosuccinic acid, anserine, 2-amino-adipic acid, 3-amino-isobutyric acid, beta-alanine, ethanolamine, phenylalanine, phosphoserine, o-phosphroyl ethanolamine, gamma aminobutyric acid, glutamic acid, glycine, glutamine, hydroxyproline, homocitrulline, homocysteine, histidine, hyroxylysine, isoleucine, carnosine, lysine, leucine, methionine, 3-methylhistidine, 1-methylhistidine, norvaline, ornithine, proline, cystine, cystathionine, sarcosine, citrulline, serine, taurine, tryptophan, tyrosine, threonine, valine

  • MatrIx

    • Serum
    • Plasma
    • Urine
Explore Results

NO

AMINO ACIDS

LOQ (nmol/ml)

Recovery (%)

Repeatability (%)

1

Tryptophane

0.23

117

3.68

2

Taurine

5.47

85

4.81

3

Phenylalanine

0.73

110

3.73

4

Tyrosine

3.34

101

4.09

5

Leucine

0.20

97

4.62

6

Isoleucine

0.22

107

1.13

7

Methionine

0.41

90

3.70

8

Gamma-aminobutyric acid

0.07

104

8.49

9

3-Amino-isobutyric acid

0.15

100

6.49

10

Norvaline

0.15

95

4.90

11

2-Amino-adipic acid

0.83

94

2.23

12

Beta alanine

1.00

90

7.22

13

Ethanolamine

0.56

106

5.79

14

Aspartic acid

1.06

96

3.96

15

L-2-aminobutyric acid

0.22

94

4.71

16

Threonine

0.72

97

3.99

17

Serine

0.89

100

5.39

18

Alanine

6.82

104

4.44

19

Glycine

9.08

101

2.24

20

Asparagine

0.46

91

3.71

21

Trans-4-hydroxy-L-proline

0.28

102

1.90

22

Glutamine

0.32

98

3.58

23

Sarcosine

4.38

113

2.16

24

Proline

0.67

100

3.66

25

Homocitrulline

0.89

105

3.50

26

Citrulline

0.51

93

3.42

27

Homocysteine

0.31

103

5.32

28

Cystine

0.97

100

4.39

29

Cystathionine

0.25

104

5.81

30

Arginine

0.15

107

3.66

31

Histidine

2.43

54

1.49

32

Ornithine

1.39

96

3.34

33

DL-5- hydroxylysine

0.30

104

1.64

34

Lysine

0.09

114

4.52

35

Carnosine

0.12

93

2.56

36

3-Methylhistidine

0.30

100

4.49

37

1-Methylhistidine

0.20

78

2.94

38

Anserine

0.21

107

3.72

39

O-phosphoserine

0.12

99

6.20

40

Glutamic acid

0.24

98

9.30

41

Valine

5.11

42

4.68

42

Argininosuccinic acid

0.62

87

3.75

43

Orthophosphoryl ethanolamine

2.56

90

2.96

44

Allo-isoleucine

0.04

101

1.51


  • Extracted chromatogram of amIno acIds-spIked serum sample

Sample Preparation for Serum, Plasma and Urine


  • Step 1

    Pipet 50 μl serum or urine sample into a centrifuge tube

  • Step 2

    Add 50 μl internal standart solution and 700 μl Reagent-1 onto sample and vortex for 10 seconds

  • Step 3

    Centrifuge at 4000 rpm for 5 minutes

  • Step 4

    Decant the supernatant into HPLC vial and inject into the system

ADAPTING DIAGNOSTICS IN CHROMATOGRAPHY COUPLED MASS SPECTROMETRY.

Contact Us

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