Homocysteine is a non-essential sulfur containing amino acid closely linked to the essential amino acid methionine. Homocysteine is rapidly oxidized in plasma into homocystine and cysteine-homocysteine disulfide. Total plasma homocysteine is the sum of homocysteine and its oxidized forms.
The determination of total homocysteine plays an indicator role in diagnosis and therapy of folate, vitamin B12 and vitamin B6 deficiencies. In addition, several observational studies have confirmed that increased total homocysteine is an independent predictor of cardiovascular risk.
Jasem method ensures straightforward analysis of homocysteine, in serum, plasma and dry blood spot (DBS) during 5.0 minutes.
Pipet 50 μL of serum/plasma sample into a glass centrifuge tube and add 50 μL of internal standard
Vortex for 5 seconds and add 100 μL of reducing solution, vortex for 15 seconds
Then, incubate in a water bath at 60°C for 15 minutes
After that add 650 μL reagent-2 and centrifuge at 3000 rpm for 5 minutes. Decant the supernatant into HPLC vial