HISTAMINE KIT

Histamine Analysis Using LC-MS/MS

Histamine is a common biogenic amine produced by microbial decarboxylation of histidine in different types of fermented foods/beverages, which include dairy products, beer, wine, fish, sausages, and other processed meats. Therefore histamine is an indicator of deterioration in food, and is frequently regarded as one of the biomarkers for quality control during the food production and transportation.

Histamine plays crucial roles mediating the immune response, regulating blood vessel (high concentration of histamine has strong vasoactive effects in humans) and controlling intestinal smooth muscle motility. The most well-known role of histamine is mediating part of the inflammatory response to various allergic reactions. Histamine in food can induce both toxicological risk and health risk.

Jasem method provides straightforward analysis of histamine, in trachurus, orkinos, cheese, wine, salami, beer and soya sauce using LC/MS-MS during 7.5 minutes

  • Features

    • No need On-line SPE
    • Use of only one standard HPLC system and one column 
    • Quick and easy sample preparation 
    • Short analysis time (7. 5 min)
  • Parameters

    Histamine
  • MATRIX

    Wine

    Beer

    Soya Sauce

    Cheese

    Trachurus

    Orkinos

    Salami

Sample Preparation for Various Food Types


  • Step 1

    Transfer accurately 5 g sample into a centifuge tube

  • Step 2

    Add 20 ml of Reagent-1 and vortex 10 minutes vigorously

  • Step 3

    Centrifuge at 4000 rpm (+4°C) for 10 minutes

  • Step 4

    Filter out 0.45 micron nylon filters into a HPLC vial

  • Extracted Ion Chromatogram / Results

     

    Matrix

    LOQ (ppm)

    Trachurus

    1.06

    Orkinos

    2.41

    Cheese

    0.92

    Salami

    1.16

    Soya Sauce

    1.48

    Beer

    1.00

    Wine

    4.32

  • Calıbratıon Curve of Hıstamıne ın Orkınos Matrıx

     

ADAPTING DIAGNOSTICS IN CHROMATOGRAPHY COUPLED MASS SPECTROMETRY.

Contact Us

The easiest way is to fill the contact form. Simply mail us to info@jasem.com.tr or give us a call +902165720200