STEROID HORMONES KIT

Steroid Hormones Analysis in Serum

Steroid hormones are defined as effective biological messengers which take part in crucial body processes even at low concentrations. Thus, dependable and simultaneous analysis of a broad panel of steroid hormones plays vital role for the investigation of the hormone profile, which could be useful in detection endocrine disorders resulting from defects in steroid biosynthesis such as congenital adrenal hyperplasia (CAH). Due to the restrictions and shortcomings of immunoassay techniques for measurement of steroid hormones in body fluids, liquid chromatography tandem mass spectrometry (LC-MS/MS) is increasingly becoming the method for multi-class steroid hormone detection.

Jasem’s approach is offering simultaneously quantification of clinically prominent 16 steroid hormones by LC-MS/MS in 16 minutes.

  • Features

    • Using a simple liquid–liquid-extraction (LLE) protocol for preparation of specimen and no derivatization is needed
    • Detection of multiple target analytes in a single run
    • Measurement of multi-class steroids without sacrificing analysis time
    • Ensure accurate quantitation, high sensitivity and selectivity comparing to immunoassays
  • Parameters

    • 17-α-Hydroxyprogesterone
    • Aldosterone
    • Testosterone
    • Estradiol
    • Progesterone
    • Cortisol
    • Dihydrotestosterone
    • Androstene-3,17-dione
    • Corticosterone
    • 11-Deoxycortisol
    • Pregnenolone
    • 11-Deoxycorticosterone
    • DHEA
    • DHEAS
    • 17-α-Hydroxypregnenolone
    • Androsterone.
  • MATRIX

    • Serum

Sample Preparation for Serum


  • Step 1

    Pipet 225 μl of serum sample into a glass centrifuge tube

  • Step 2

    Add 25 μl of labeled-internal standard mixture and vortex for 5 seconds. Add 450 μl of Reagent-1 in the serum sample and additionally vortex for 15 seconds. Centrifuge at 4000 rpm for 5 minutes

  • Step 3

    Decant the clear supernatant into a new glass centrifuge tube and wash the precipitated tube with 1.5 mL Reagent-2. After liquid phases are combined and shake for 3 minutes. Then centrifuge at 4000 rpm for 5 minutes

  • Step 4

    The upper phase is evaporated under nitrogen stream and dissolved with 100 μl Reagent-3. Transfer the final solution into the HPLC vial

  • Extracted LC-MS/MS chromatogram of Steroıd Hormones

  • Result

    Steroid Hormone

    LOQ (µg/L)

    Recoveries (%)

    Repeatability (%)

    Aldosterone

    0.035

    95.8

    6.33

    Cortisol

    0.336

    91.6

    2.48

    Corticosterone

    0.179

    102.3

    4.96

    11-Deoxycortisol

    0.048

    108.4

    3.62

    DHEAS

    16.32

    105.9

    2.97

    Estradiol

    0.051

    108.7

    5.99

    Testosterone

    0.026

    93.4

    1.88

    DHEA

    0.336

    109.2

    8.57

    11-Deoxycorticosterone

    0.052

    95.8

    7.73

    Androstene-3,17-dione

    0.076

    97.4

    2.47

    17-α-Hydroxyprogesterone

    0.091

    97.2

    4.31

    Dihydrotestosterone

    0.041

    104.0

    9.92

    Androsterone

    0.115

    87.8

    9.39

    Pregnenolone

    0.108

    92.4

    7.53

    17-α-Hydroxypregnenolone

    0.480

    99.3

    6.55

    Progesterone

    0.108

    113.9

    7.11

ADAPTING DIAGNOSTICS IN CHROMATOGRAPHY COUPLED MASS SPECTROMETRY.

Contact Us

The easiest way is to fill the contact form. Simply mail us to info@jasem.com.tr or give us a call +902165720200