25-Hydroxy Vıtamın D2/D3 kıt

25-Hydroxy Vitamin D2/D3 in Serum/Plasma Using LC-MS/MS

Vitamin D plays a vital role in skeletal metabolism, calcium homeostasis, and also in the functioning of the immune, cardiovascular, and reproductive systems. Vitamin D deficiency leads to rickets and osteomalacia and is also associated with breast and colorectal cancers, multiple sclerosis, dementia, rheumatoid arthritis, diabetes, Parkinson’s and Alzheimer’s diseases.

Consequently, laboratory testing of serum vitamin D levels has experienced phenomenal growth over the past several years. LC-MS/MS is currently the best technique available for the correct quantification of 25-Hydroxy Vitamin D3 and 25-Hydroxy Vitamin D2.Jasem method provides analysis of 25-Hydroxyvitamin D3, 25-Hydroxyvitamin D2 and 3-epi-25-Hydroxyvitamin D3 in serum samples using simple sample preparation technique without SPE, on-line SPE or evaporation.

  • Features

    • No need SPE,  on-line SPE  or evaporation
    • Use of only one standard HPLC system and one column
    • Quick and easy sample preparation
    • Sufficient chromatographic separation of 3-epi-25-hydroxy vitamin D3 in 8.5 minutes with accurate quantification
  • Parameters

    25-Hydroxy vıtamın D2, 25-Hydroxy vıtamın D3, 3-epı-25-Hydroxy vıtamin D3
  • Matrıx

    • Serum
    • Plasma
  • VITAMIN D ANALYSIS RESULT

     

    LOQ (μg/L)

    Recovery (%)

    Repeatability (%)

    25-OH Vitamin D3

    1.52

    93

    2.81

    25-OH Vitamin D2

    3.70

    113

    2.72

  • Extracted chromatogram of 25-OH Vıtamın D2, 25-OH Vıtamın D3, 3-epı-25-OH Vıtamın D3

Sample Preparation for Serum / Plasma


  • Step 1

    Pipet 200 μL serum sample into a centrifuge tube

  • Step 2

    Add 100 μL of Reagent-1 and 25 μL of IS on sample and vortex for 15 seconds

  • Step 3

    Add 975 μL of Reagent-2 and vortex for an additional 15 seconds

  • Step 4

    Centrifuge at 4000 rpm for 5 minutes

  • Step 5

    Decant the supernatant into HPLC vial and inject into the system

ADAPTING DIAGNOSTICS IN CHROMATOGRAPHY COUPLED MASS SPECTROMETRY.

Contact Us

The easiest way is to fill the contact form. Simply mail us to info@jasem.com.tr or give us a call +902165720200